HAL-2/2

General Information


DCTPep ID  DCTPep00359

Peptide Name   HAL-2/2

Sequence  GKWMKLLKHILK

Sequence Length  12

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
SW480 Colon adenocarcinoma IC50=35±5 µM MTT assay 48 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=6±1 µM MTT assay 48 h 1
CCRF-CEM Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia IC50>40 µM MTT assay 48 h 1

Hemolytic Activity  Rat erythrocytes: IC50=87.4 μM

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50=40±4 μM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C72H123N19O13S1

Absent amino acids  ACDEFNPQRSTVY

Theoretical pI  10.48

Acidic residues  0

Basic residues  5

Polar residues  1

Molecular weight (Average)  1494.95

Molecular weight (Monoisotopic)  1493.93

Common amino acids  K

Net charge  5

Instability index (II)  53.27

Aliphatic index  130.00

Grand average of hydropathicity (GRAVY)  -0.192

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 3.679

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 22100226

Title  Toxicity study of antimicrobial peptides from wild bee venom and their analogs toward mammalian normal and cancer cells

Doi 10.1016/j.peptides.2011.11.002

Year  2012

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.