Peptide Libraray

HAL-1/15

General Information

DCTPep ID DCTPep00360

Peptide Name HAL-1/15

Sequence GMWSKLLGHLLR

Sequence Length 12

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HeLa	Human papillomavirus-related endocervical adenocarcinoma	IC50=14±2 µM	MTT assay	48 h	1
SW480	Colon adenocarcinoma	IC50> 40 µM	MTT assay	48 h	1
CCRF-CEM	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50>40 µM	MTT assay	48 h	1

Hemolytic Activity Rat erythrocytes: IC50=62 μM

Normal (non-cancerous) Cytotoxicity HUVEC: IC50=26±6 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C65H107N19O14S1

Absent amino acids ACDEFINPQTVY

Theoretical pI 11.00

Acidic residues 0

Basic residues 3

Polar residues 3

Molecular weight (Average) 1410.74

Molecular weight (Monoisotopic) 1409.8

Common amino acids L

Net charge 3

Instability index (II) 18.14

Aliphatic index 130.00

Grand average of hydropathicity (GRAVY) 0.250

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.899

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 22100226

Title Toxicity study of antimicrobial peptides from wild bee venom and their analogs toward mammalian normal and cancer cells

Doi 10.1016/j.peptides.2011.11.002

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available