Peptide Libraray

LL-III

General Information

DCTPep ID DCTPep00375

Peptide Name LL-III

Sequence VNWKKILGKIIKVVK

Sequence Length 15

UniProt ID Not available

PubChem CID Not available

Origin Lasioglossin III and its analogs

Type Native peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
SW480	Colon adenocarcinoma	IC50=18±4 µM	MTT assay	48 h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	IC50=4±1 µM	MTT assay	48 h	1
CCRF-CEM	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=5±3 µM	MTT assay	48 h	1

Hemolytic Activity Rat erythrocytes: IC50>200 μM

Normal (non-cancerous) Cytotoxicity HUVEC: IC50=15±4 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00375

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C86H152N22O17

Absent amino acids ACDEFHMPQRSTY

Theoretical pI 10.60

Acidic residues 0

Basic residues 5

Polar residues 2

Molecular weight (Average) 1766.29

Molecular weight (Monoisotopic) 1765.17

Common amino acids K

Net charge 5

Instability index (II) -14.96

Aliphatic index 162.00

Grand average of hydropathicity (GRAVY) 0.373

Half Life
100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.114

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 22100226

Title Toxicity study of antimicrobial peptides from wild bee venom and their analogs toward mammalian normal and cancer cells

Doi 10.1016/j.peptides.2011.11.002

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available