Chassatide C2

General Information


DCTPep ID  DCTPep00397

Peptide Name   Chassatide C2

Sequence  GIPCAESCVWIPCTITALMGCSCKNNVCYNN

Sequence Length  31

UniProt ID  I0B6F2 

PubChem CID  Not available

Origin  Chassalia chartacea

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=2.4 μM MTT assay Not available 1

Hemolytic Activity  Human erythrocytes: HD50>25 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00397

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys4<--->Cys21; Cys8<--->Cys23; Cys13<--->Cys28; NCB: Gly1<--->Asn31

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C138H219N37O43S7

Absent amino acids  DFHQR

Theoretical pI  5.96

Acidic residues  1

Basic residues  1

Polar residues  17

Molecular weight (Average)  3308.9

Molecular weight (Monoisotopic)  3306.41

Common amino acids  C

Net charge  0

Instability index (II)  35.55

Aliphatic index  75.48

Grand average of hydropathicity (GRAVY)  0.503

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 7365
  Abs 0.1% (=1 g/l) 2.226, assuming all pairs of Cys residues form cystines
  Ext. coefficient 6990
  Abs 0.1% (=1 g/l) 2.112, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 22467870

Title  Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities

Doi 10.1074/jbc.M111.338970

Year  2012

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_4283

DCTPep is developed by Dr.Zheng's team.