GA-K3

General Information


DCTPep ID  DCTPep00408

Peptide Name   GA-K3

Sequence  FLGWLFKWAKK

Sequence Length  11

UniProt ID  Not available

PubChem CID  Not available

Origin  Brevinin-1EMa

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
SK-N-DZ Neuroblastoma IC50=24.76 µM MTT assay 72 h 1
A549 Lung adenocarcinoma IC50=25.20 µM MTT assay 72 h 1
MKN45 Gastric adenocarcinoma IC50=26.70 µM MTT assay 72 h 1
HCT 116 Colon carcinoma IC50=27.00 µM MTT assay 72 h 1
SK-MEL-2 Melanoma IC50=27.39 µM MTT assay 72 h 1
A498 Renal cell carcinoma IC50=41.21 µM MTT assay 72 h 1
PC-3 Prostate carcinoma IC50=47.69 µM MTT assay 72 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C75H106N16O12

Absent amino acids  CDEHIMNPQRSTVY

Theoretical pI  10.30

Acidic residues  0

Basic residues  3

Polar residues  1

Molecular weight (Average)  1423.77

Molecular weight (Monoisotopic)  1422.82

Common amino acids  K

Net charge  3

Instability index (II)  4.2

Aliphatic index  80.00

Grand average of hydropathicity (GRAVY)  0.100

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 11000
  Abs 0.1% (=1 g/l) 7.726

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 22644847

Title  Anticancer activity of undecapeptide analogues derived from antimicrobial peptide, brevinin-1EMa

Doi 10.1007/s12272-012-0505-0

Year  2012

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.