Peptide Libraray

General Information

DCTPep ID DCTPep00444

Peptide Name 9R

Sequence RRRRRNWMWC

Sequence Length 10

UniProt ID Not available

PubChem CID Not available

Origin Nullomer derived anticancer peptides (NulloPs):

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
J774A.1	Mouse reticulum cell sarcoma	IC50=12 µM	MTT assay	96 h	1
MDA-MB-231	Breast adenocarcinoma	IC50=16 µM	MTT assay	48 h	1
LNCaP	Prostate carcinoma	IC50=23 µM	MTT assay	24 h	1
HuT 102	Mycosis fungoides; Classic mycosis fungoides	IC50=25 µM	MTT assay	96 h	1
LNCaP	Prostate carcinoma	IC50=28 µM	MTT assay	48 h	1
MDA-MB-231	Breast adenocarcinoma	IC50=29 µM	MTT assay	24 h	1
HuT 102	Mycosis fungoides; Classic mycosis fungoides	IC50=36 µM	MTT assay	72 h	1
HuT 102	Mycosis fungoides; Classic mycosis fungoides	IC50=37 µM	MTT assay	48 h	1
HuT 102	Mycosis fungoides; Classic mycosis fungoides	IC50=39 µM	MTT assay	24 h	1
MDA-MB-231	Breast adenocarcinoma	IC50=39 µM	MTT assay	2 h	1
LNCaP	Prostate carcinoma	IC50=44 µM	MTT assay	2 h	1
J774A.1	Mouse reticulum cell sarcoma	IC50=47 µM	MTT assay	24 h	1
HuT 102	Mycosis fungoides; Classic mycosis fungoides	IC50=93 µM	MTT assay	2 h	1

Hemolytic Activity no hemolytic effect on human RBCs

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C64H102N28O12S2

Absent amino acids ADEFGHIKLPQSTVY

Theoretical pI 12.18

Acidic residues 0

Basic residues 5

Polar residues 2

Molecular weight (Average) 1519.81

Molecular weight (Monoisotopic) 1518.77

Common amino acids R

Net charge 5

Instability index (II) 263.77

Aliphatic index 0.00

Grand average of hydropathicity (GRAVY) -2.340

Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 7.238, assuming all pairs of Cys residues form cystines
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 7.238, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 23000474

Title Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro

Doi 10.1016/j.peptides.2012.09.015

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available