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Ranalexin
General Information
DCTPep ID DCTPep00447
Peptide Name Ranalexin
Sequence FLGGLIKIVPAMICAVTKKC
Sequence Length 20
UniProt ID P39084
PubChem CID Not available
Origin Rana catesbeiana
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=13.4 µg/ml | MTT assay | 24h | 1 |
| LNCaP C4-2 | Prostate carcinoma | IC50=30 µg/ml | MTT assay | 24 h | 2 |
| MEC-7 | Invasive breast carcinoma of no special type | IC50=30 µg/ml | MTT assay | 24 h | 2 |
| HepG2 | Hepatoblastoma | IC50=31 µg/ml | MTT assay | 24 h | 2 |
Hemolytic Activity Human erythrocytes: <15% Hemolysis=250 µg/ml
Normal (non-cancerous) Cytotoxicity Monkey COS-7: IC50=14.8 µg/ml; NL20: Not active up to 62.5 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00447
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys14<--->Cys20
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C97H169N23O22S3
Absent amino acids DEHNQRSWY
Theoretical pI 9.39
Acidic residues 0
Basic residues 3
Polar residues 5
Molecular weight (Average) 2105.73
Molecular weight (Monoisotopic) 2104.2
Common amino acids IK
Net charge 3
Instability index (II) 17.95
Aliphatic index 136.50
Grand average of hydropathicity (GRAVY) 1.400
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.059, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23053091
Title Molecular cloning and expression of ranalexin, a bioactive antimicrobial peptide from Rana catesbeiana in Escherichia coli and assessments of its biological activities
Year 2013
Literature 2
Pubmed ID 29649587
Title A novel tool against multiresistant bacterial pathogens: lipopeptide modification of the natural antimicrobial peptide ranalexin for enhanced antimicrobial activity and improved pharmacokinetics
Doi 10.1016/j.ijantimicag.2018.03.023
Year 2018
Literature 3
Pubmed ID 26046345
Title Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae
Doi 10.1371/journal.pone.0128532
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.