Temporin-1CEa LK3

General Information


DCTPep ID  DCTPep00459

Peptide Name   Temporin-1CEa LK3

Sequence  FKKLKKIANIINSIFKK

Sequence Length  17

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic construct

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MDA-MB-231 Breast adenocarcinoma IC50=224.8µM MTT assay 24h 1
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma IC50=25.04µM MTT assay 24h 1
MCF-7 Invasive breast carcinoma of no special type IC50=27.72µM MTT assay 24h 1

Hemolytic Activity  Human erythrocytes: LC50=4649 µM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00459

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C98H169N25O21

Absent amino acids  CDEGHMPQRTVWY

Theoretical pI  10.70

Acidic residues  0

Basic residues  6

Polar residues  3

Molecular weight (Average)  2033.57

Molecular weight (Monoisotopic)  2032.29

Common amino acids  K

Net charge  6

Instability index (II)  2.59

Aliphatic index  120.59

Grand average of hydropathicity (GRAVY)  -0.118

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 23609760

Title  Design of potent, non-toxic anticancer peptides based on the structure of the antimicrobial peptide, temporin-1CEa

Doi 10.1007/s12272-013-0112-8

Year  2013

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_4540

DCTPep is developed by Dr.Zheng's team.