This work is licensed under a Creative Commons Attribution 4.0 International License.
NRC-16
General Information
DCTPep ID DCTPep00465
Peptide Name NRC-16
Sequence GWKKWLRKGAKHLGQAAIK
Sequence Length 19
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | 50% Cell death=32 µg/ml | MTT assay | 2h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>256 µg/ml
Normal (non-cancerous) Cytotoxicity HaCat: Not active up to 50 µM; Zebrafish embryos: >10% Killing=5 µM(52hpf embryos (hpf - hours postfertilization)); Zebrafish embryos: >10% Killing=25 µM(4hpf, 28hpf embryos)
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00465
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C102H166N32O21
Absent amino acids CDEFMNPSTVY
Theoretical pI 11.39
Acidic residues 0
Basic residues 7
Polar residues 3
Molecular weight (Average) 2176.64
Molecular weight (Monoisotopic) 2175.29
Common amino acids K
Net charge 7
Instability index (II) 19.19
Aliphatic index 77.37
Grand average of hydropathicity (GRAVY) -0.853
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 5.054
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21729875
Title The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents
Year 2011
Literature 2
Pubmed ID 23760014
Title Anti-microbial, anti-biofilm activities and cell selectivity of the NRC-16 peptide derived from witch flounder, Glyptocephalus cynoglossus
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.