Peptide Libraray

Melittin

General Information

DCTPep ID DCTPep00468

Peptide Name Melittin

Sequence GIGAVLKVLTTGLPALISWIKRKRQQ

Sequence Length 26

UniProt ID P68407 P01501 Q8LW54

PubChem CID 16133648

Origin Apis mellifera (Honeybee)

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
E42/02	Horse sarcoid	IC50=1.4 ± 0.1 µM	MTT assay	24 h	1
E42/02	Horse sarcoid	IC50=1.8 ± 0.1 µM	MTT assay	4 h	1
E42/02	Horse sarcoid	IC50=2.1 ± 0.1 µM	MTT assay	2 h	1
E42/02	Horse sarcoid	IC50=3.2 ± 0.1 µM	MTT assay	30 min	1
PC-3	Prostate carcinoma	LD50=13 µg/ml	MTT assay	24 h	Patent
BMKC	Skin carcinoma	LD50=18 µg/ml	MTT assay	24 h	Patent
SW480	Colon adenocarcinoma	LD50=23 µg/ml	MTT assay	24 h	Patent
NCI-H1299	Lung large cell carcinoma	LD50=25 µg/ml	MTT assay	24 h	Patent
HeLa	Human papillomavirus-related endocervical adenocarcinoma	LD50=35 µg/ml	MTT assay	24 h	Patent
MCF-7	Invasive breast carcinoma of no special type	LD50=9 µg/ml	MTT assay	24 h	Patent

Hemolytic Activity high haemolytic activity

Normal (non-cancerous) Cytotoxicity APH-R: IC50=0.9±0.1 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID 1BH1

Predicted Structure DCTPep00468

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C131H228N38O32

Absent amino acids CDEFHMNY

Theoretical pI 12.02

Acidic residues 0

Basic residues 5

Polar residues 6

Molecular weight (Average) 2847.49

Molecular weight (Monoisotopic) 2845.74

Common amino acids L

Net charge 5

Instability index (II) 44.73

Aliphatic index 135.00

Grand average of hydropathicity (GRAVY) 0.273

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.932

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 23893605

Title Design of NK-2-derived peptides with improved activity against equine sarcoid cells

Doi 10.1002/psc.2540

Year 2013

Patent

Patent ID US2005/0209157A1

Patent Title Short bioactive peptides and methods for their use

Other Iinformation Patent Application; Family: 1s / 24ex; Family Jurisdictions: US; Legal Status: Discontinued; Application No: 13618605; Filed: May 24, 2005; Published: Sep 22, 2005; Earliest Priority: Mar 28, 2001

Other Published ID Not available

External Code

DBAASP ID DBAASPR_806