This work is licensed under a Creative Commons Attribution 4.0 International License.
KilerFLIP-E
General Information
DCTPep ID DCTPep00477
Peptide Name KilerFLIP-E
Sequence YGRKKRRQRRRREADFFWSLCTA
Sequence Length 23
UniProt ID Not available
PubChem CID Not available
Origin The C-terminal domain of c-FLIP
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| LNCaP | Prostate carcinoma | At 20 μM, about 30% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| PC-3-TR | Prostate carcinoma | At 20 μM, about 30% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| RS4;11 | Adult B acute lymphoblastic leukemia; B acute lymphoblastic leukemia with t(v;11q23.3) MLL rearranged; B-lymphoblastic leukemia/lymphoma with t(v;11q23.3) | At 20 μM, 10.4% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| MV4-11 | Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia | At 20 μM, 10.8% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| HCT 115 | Human colon cancer | At 20 μM, 40% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | At 20 μM, 7.7% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| PC-3 | Prostate carcinoma | At 20 μM, about 50% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
| HCT 116 | Colon carcinoma | At 20 μM, about 70% Trypan blue-negative cells | Trypan blue assay | 18 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity No toxicity was observed in epithelial and endothelial cells (HPDE and HUVEC, respectively)
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00477
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C130H208N48O32S1
Absent amino acids HIMNPV
Theoretical pI 11.62
Acidic residues 2
Basic residues 9
Polar residues 5
Molecular weight (Average) 2987.44
Molecular weight (Monoisotopic) 2985.58
Common amino acids R
Net charge 7
Instability index (II) 135.24
Aliphatic index 25.65
Grand average of hydropathicity (GRAVY) -1.670
Half Life
2.8 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.340, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.340, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24176852
Title killerFLIP: a novel lytic peptide specifically inducing cancer cell death
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.