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Short α-helical peptides
General Information
DCTPep ID DCTPep00479
Peptide Name Short α-helical peptides
Sequence GIIKKIIKKIIKKI
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Alpha-helical proteins
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | 90% Cytotoxicity at 5 µM Approx | MTT assay | 24 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 95% Cytotoxicity at 4 µM Approx | MTT assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: HC50>250 µM
Normal (non-cancerous) Cytotoxicity Human keratinocytes HaCat: <5% Killing=40 µM
Target Not available
Affinity Not available
Mechanism In addition to rapid membrane-permeabilizing capacities, the peptides can also induce the programmed cell death of cancer cells via both mitochondrial pathway and death receptor pathway, without inducing non-specific immunogenic responses.
Structure Information
PDB ID Not available
Predicted Structure DCTPep00479
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C80H154N20O15
Absent amino acids ACDEFHLMNPQRSTVWY
Theoretical pI 10.70
Acidic residues 0
Basic residues 6
Polar residues 1
Molecular weight (Average) 1636.23
Molecular weight (Monoisotopic) 1635.19
Common amino acids I
Net charge 6
Instability index (II) -33.16
Aliphatic index 195.00
Grand average of hydropathicity (GRAVY) 0.550
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24246647
Title Molecular mechanisms of anticancer action and cell selectivity of short α-helical peptides
Doi 10.1016/j.biomaterials.2013.10.082
Year 2014
Literature 2
Pubmed ID Not available
Title Amino acid side chains affect the bioactivity of designed short peptide amphiphiles
Year 2016
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.