Short α-helical peptides

General Information


DCTPep ID  DCTPep00480

Peptide Name   Short α-helical peptides

Sequence  GIIKKIIKKIIKKIIKKI

Sequence Length  18

UniProt ID  Not available

PubChem CID  Not available

Origin  Alpha-helical proteins

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HL-60 Adult acute myeloid leukemia; Acute myeloid leukemia 70% Cytotoxicity at 5 µM Approx MTT assay 24 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma 80% Cytotoxicity at 4 µM Approx MTT assay 24 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  In addition to rapid membrane-permeabilizing capacities, the peptides can also induce the programmed cell death of cancer cells via both mitochondrial pathway and death receptor pathway, without inducing non-specific immunogenic responses.



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00480

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C104H200N26O19

Absent amino acids  ACDEFHLMNPQRSTVWY

Theoretical pI  10.85

Acidic residues  0

Basic residues  8

Polar residues  1

Molecular weight (Average)  2118.89

Molecular weight (Monoisotopic)  2117.55

Common amino acids  I

Net charge  8

Instability index (II)  -33.01

Aliphatic index  195.00

Grand average of hydropathicity (GRAVY)  0.494

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 24246647

Title  Molecular mechanisms of anticancer action and cell selectivity of short α-helical peptides

Doi 10.1016/j.biomaterials.2013.10.082

Year  2014

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.