Peptide Libraray

Short α-helical peptides

General Information

DCTPep ID DCTPep00480

Peptide Name Short α-helical peptides

Sequence GIIKKIIKKIIKKIIKKI

Sequence Length 18

UniProt ID Not available

PubChem CID Not available

Origin Alpha-helical proteins

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HL-60	Adult acute myeloid leukemia; Acute myeloid leukemia	70% Cytotoxicity at 5 µM Approx	MTT assay	24 h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	80% Cytotoxicity at 4 µM Approx	MTT assay	24 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism In addition to rapid membrane-permeabilizing capacities, the peptides can also induce the programmed cell death of cancer cells via both mitochondrial pathway and death receptor pathway, without inducing non-specific immunogenic responses.

Structure Information

PDB ID Not available

Predicted Structure DCTPep00480

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C104H200N26O19

Absent amino acids ACDEFHLMNPQRSTVWY

Theoretical pI 10.85

Acidic residues 0

Basic residues 8

Polar residues 1

Molecular weight (Average) 2118.89

Molecular weight (Monoisotopic) 2117.55

Common amino acids I

Net charge 8

Instability index (II) -33.01

Aliphatic index 195.00

Grand average of hydropathicity (GRAVY) 0.494

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 24246647

Title Molecular mechanisms of anticancer action and cell selectivity of short α-helical peptides

Doi 10.1016/j.biomaterials.2013.10.082

Year 2014

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available