This work is licensed under a Creative Commons Attribution 4.0 International License.
BP76, Cecropin A (2-8)[W2K] + Melittin (6-9)[V8F]
General Information
DCTPep ID DCTPep00484
Peptide Name BP76, Cecropin A (2-8)[W2K] + Melittin (6-9)[V8F]
Sequence KKLFKKILKFL
Sequence Length 11
PubChem CID 60144929
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Cell-penetrating peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| CAPAN-1 | Pancreatic ductal adenocarcinoma | IC50=23.5±7.0 µM | MTT assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=26.3±7.5 µM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=203 µM; 34 ±2.1% Hemolysis=150 µM
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=13.5±0.7 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C72H124N16O12
Absent amino acids ACDEGHMNPQRSTVWY
Theoretical pI 10.60
Acidic residues 0
Basic residues 5
Polar residues 0
Molecular weight (Average) 1405.88
Molecular weight (Monoisotopic) 1404.96
Common amino acids K
Net charge 5
Instability index (II) -10.22
Aliphatic index 141.82
Grand average of hydropathicity (GRAVY) 0.182
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24480922
Title Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties
Year 2014
Literature 2
Pubmed ID 16672470
Title Inhibition of plant-pathogenic bacteria by short synthetic cecropin A-melittin hybrid peptides
Doi 10.1128/AEM.72.5.3302-3308.2006
Year 2006
Literature 3
Pubmed ID 19617390
Title Sporicidal activity of synthetic antifungal undecapeptides and control of Penicillium rot of apples
Year 2009
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_915
This work is licensed under a Creative Commons Attribution 4.0 International License.