This work is licensed under a Creative Commons Attribution 4.0 International License.
Dermaseptin S4 [M4D][N20D]
General Information
DCTPep ID DCTPep00485
Peptide Name Dermaseptin S4 [M4D][N20D]
Sequence ALWDTLLKKVLKAAAKAALDAVLVGANA
Sequence Length 28
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 50% Cytotoxicity=24.12 µg/ml | MTT assay | 0.5, 3, 6h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=5±1 µM
Normal (non-cancerous) Cytotoxicity Vero cells: 50% Cytotoxicity =17.75 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00485
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C131H224N34O35
Absent amino acids CEFHIMPQRSY
Theoretical pI 9.53
Acidic residues 2
Basic residues 4
Polar residues 3
Molecular weight (Average) 2835.43
Molecular weight (Monoisotopic) 2833.68
Common amino acids A
Net charge 2
Instability index (II) -2.43
Aliphatic index 146.79
Grand average of hydropathicity (GRAVY) 0.839
Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.940
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24492362
Title In vitro activities of dermaseptins K4S4 and K4K20S4 against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa planktonic growth and biofilm formation
Year 2014
Literature 2
Pubmed ID 10660589
Title Structure-activity relationship study of antimicrobial dermaseptin S4 showing the consequences of peptide oligomerization on selective cytotoxicity
Year 2000
Literature 3
Pubmed ID 23161023
Title In vitro antiviral activity of dermaseptin S(4) and derivatives from amphibian skin against herpes simplex virus type 2
Year 2013
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.