Peptide Libraray

Cathelicidin-RC1

General Information

DCTPep ID DCTPep00495

Peptide Name Cathelicidin-RC1

Sequence KKCKFFCKVKKKIKSIGFQIPIVSIPFK

Sequence Length 28

UniProt ID Not available

PubChem CID Not available

Origin Lithobates catesbeiana

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
PC-3	Prostate carcinoma	At 200 µg/ml induced 2.5% cell death	MTT assay	48h	1
HepG2	Hepatoblastoma	At 200 µg/ml induced 4.3% cell death	MTT assay	48h	1

Hemolytic Activity Human erythrocytes:0% Hemolysis=100µg/ml; 3% Hemolysis=200µg/ml

Normal (non-cancerous) Cytotoxicity L929: At 200 µg/ml induced 1.1% cell death

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00495

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C159H264N38O32S2

Absent amino acids ADEHLMNRTWY

Theoretical pI 10.32

Acidic residues 0

Basic residues 9

Polar residues 5

Molecular weight (Average) 3284.2

Molecular weight (Monoisotopic) 3281.96

Common amino acids K

Net charge 9

Instability index (II) -4.06

Aliphatic index 90.36

Grand average of hydropathicity (GRAVY) 0.118

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.038, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 24675879

Title Cathelicidins from the bullfrog Rana catesbeiana provides novel template for peptide antibiotic design

Doi 10.1371/journal.pone.0093216

Year 2014

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available