This work is licensed under a Creative Commons Attribution 4.0 International License.
C7A-D21K
General Information
DCTPep ID DCTPep00506
Peptide Name C7A-D21K
Sequence KILRGVAKKIMRTFLRRISKKILTGKK
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | Cell viability >40% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC24 | Colon adenocarcinoma | Cell viability >50% at 25μM approx | Cell Viability assay | 1 h | 1 |
| HROC40 | Colon adenocarcinoma | Cell viability >50% at 25μM approx | Cell Viability assay | 1 h | 1 |
| HROC60 | Colon adenocarcinoma | Cell viability >50% at 25μM approx | Cell Viability assay | 1 h | 1 |
| HROC18 | Cecum adenocarcinoma | Cell viability >55% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC24 | Colon adenocarcinoma | Cell viability >55% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC60 | Colon adenocarcinoma | Cell viability >60% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC107 | Colon adenocarcinoma | Cell viability >65% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC113 | Colon adenocarcinoma; Lynch syndrome | Cell viability >65% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC32 | Colon adenocarcinoma | Cell viability >80% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HROC40 | Colon adenocarcinoma | Cell viability >80% at 12.5μM approx | Cell Viability assay | 24 h | 1 |
| HCT 116 | Colon carcinoma | Cell viability=50% at 12.5μM approx. | Cell Viability assay | 1 h | 1 |
| HROC32 | Colon adenocarcinoma | Cell viability=50% at 12.5μM approx. | Cell Viability assay | 1 h | 1 |
Hemolytic Activity Whole blood cells: experiments revealed absent or minimal hemolysis
Normal (non-cancerous) Cytotoxicity lymphotoxic effects in vitro
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00506
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C145H268N46O31S1
Absent amino acids CDEHNPQWY
Theoretical pI 12.49
Acidic residues 0
Basic residues 11
Polar residues 5
Molecular weight (Average) 3184.07
Molecular weight (Monoisotopic) 3182.05
Common amino acids K
Net charge 11
Instability index (II) 42.29
Aliphatic index 115.56
Grand average of hydropathicity (GRAVY) -0.304
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 24962950
Title Host defense peptides for treatment of colorectal carcinoma - a comparative in vitro and in vivo analysis
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.