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Hymenochirin-1Pa
General Information
DCTPep ID DCTPep00511
Peptide Name Hymenochirin-1Pa
Sequence LKLSPKTKDTLKKVLKGAIKGAIAIASMA
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin skin secretions of the frog Pseudhymenochirus merlini (Pipidae)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | LC50=1.2±0.2 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=12.3±4.7 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=3.1±0.2 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
Hemolytic Activity Human red blood cells (RBC): LC50=162±13 μM
Normal (non-cancerous) Cytotoxicity HUVEC: LC50=7.7±0.7 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00511
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C136H248N36O36S1
Absent amino acids CEFHNQRWY
Theoretical pI 10.40
Acidic residues 1
Basic residues 7
Polar residues 6
Molecular weight (Average) 2995.74
Molecular weight (Monoisotopic) 2993.84
Common amino acids K
Net charge 6
Instability index (II) -5.4
Aliphatic index 121.38
Grand average of hydropathicity (GRAVY) 0.262
Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25241629
Title Conformational analysis and cytotoxic activities of the frog skin host-defense peptide, hymenochirin-1Pa
Doi 10.1016/j.peptides.2014.08.017
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.