This work is licensed under a Creative Commons Attribution 4.0 International License.
[D9K]Hymenochirin-1Pa
General Information
DCTPep ID DCTPep00512
Peptide Name [D9K]Hymenochirin-1Pa
Sequence LKLSPKTKKTLKKVLKGAIKGAIAIASMA
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin skin secretions of the frog Pseudhymenochirus merlini (Pipidae)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | LC50=0.9±0.3 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=2.4±0.1 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=3.9±0.4 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
Hemolytic Activity Human red blood cells (RBC): LC50=192±21 μM
Normal (non-cancerous) Cytotoxicity HUVEC: LC50=6.5±0.2 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00512
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C138H255N37O34S1
Absent amino acids CDEFHNQRWY
Theoretical pI 10.85
Acidic residues 0
Basic residues 8
Polar residues 6
Molecular weight (Average) 3008.83
Molecular weight (Monoisotopic) 3006.91
Common amino acids K
Net charge 8
Instability index (II) -0.21
Aliphatic index 121.38
Grand average of hydropathicity (GRAVY) 0.248
Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25241629
Title Conformational analysis and cytotoxic activities of the frog skin host-defense peptide, hymenochirin-1Pa
Doi 10.1016/j.peptides.2014.08.017
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.