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Ps-2Pa
General Information
DCTPep ID DCTPep00515
Peptide Name Ps-2Pa
Sequence GIFPIFAKLLGKVIKVASSLISKGRTE
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin skin secretions of the frog Pseudhymenochirus merlini (Pipidae)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HT-29 | Colon adenocarcinoma | LC50=11.5±2.6 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| A549 | Lung adenocarcinoma | LC50=6.0±0.6 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=6.2±0.6 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: LC50=6.2±1.0 μM
Normal (non-cancerous) Cytotoxicity HUVEC: LC50=68±2 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00515
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C135H230N34O34
Absent amino acids CDHMNQWY
Theoretical pI 10.46
Acidic residues 1
Basic residues 5
Polar residues 7
Molecular weight (Average) 2873.52
Molecular weight (Monoisotopic) 2871.73
Common amino acids IK
Net charge 4
Instability index (II) 9.02
Aliphatic index 130.00
Grand average of hydropathicity (GRAVY) 0.648
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25447194
Title Anti-cancer, immunoregulatory, and antimicrobial activities of the frog skin host-defense peptides pseudhymenochirin-1Pb and pseudhymenochirin-2Pa
Doi 10.1016/j.regpep.2014.11.001
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.