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Ps-1Pb
General Information
DCTPep ID DCTPep00516
Peptide Name Ps-1Pb
Sequence IKIPSFFRNILKKVGKEAVSLIAGALKQS
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin skin secretions of the frog Pseudhymenochirus merlini (Pipidae)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | LC50=2.5±0.2 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=6.6±0.3 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=9.5±1.3 μM | CellTiter-Glo Luminescent Cell Viability assay | 24h | 1 |
Hemolytic Activity Human red blood cells (RBC): LC50>403 μM
Normal (non-cancerous) Cytotoxicity HUVEC: LC50=5.6±0.9 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00516
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C147H251N39O37
Absent amino acids CDHMTWY
Theoretical pI 10.58
Acidic residues 1
Basic residues 6
Polar residues 6
Molecular weight (Average) 3156.85
Molecular weight (Monoisotopic) 3154.9
Common amino acids K
Net charge 5
Instability index (II) 41.25
Aliphatic index 124.48
Grand average of hydropathicity (GRAVY) 0.328
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25447194
Title Anti-cancer, immunoregulatory, and antimicrobial activities of the frog skin host-defense peptides pseudhymenochirin-1Pb and pseudhymenochirin-2Pa
Doi 10.1016/j.regpep.2014.11.001
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.