DC2

General Information


DCTPep ID  DCTPep00550

Peptide Name   DC2

Sequence  GAVPCGETCVYLPCITPDIGCSCQNKVCYRD

Sequence Length  31

UniProt ID  Not available

PubChem CID  Not available

Origin  Hedyotis diffusa

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
PC-3 Prostate carcinoma IC50=2.65 μM CCK-8 assay 72h 1
DU145 Prostate carcinoma IC50=3.11 μM CCK-8 assay 72h 1
LNCaP Prostate carcinoma IC50=4.31 μM CCK-8 assay 72h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  induce apoptosis and inhibit proliferation and migration of prostate cancer cells



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00550

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C138H219N37O45S6

Absent amino acids  FHMW

Theoretical pI  4.56

Acidic residues  3

Basic residues  2

Polar residues  15

Molecular weight (Average)  3308.84

Molecular weight (Monoisotopic)  3306.43

Common amino acids  C

Net charge  -1

Instability index (II)  12.3

Aliphatic index  69.03

Grand average of hydropathicity (GRAVY)  0.177

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 3355
  Abs 0.1% (=1 g/l) 1.014, assuming all pairs of Cys residues form cystines
  Ext. coefficient 2980
  Abs 0.1% (=1 g/l) 0.901, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 26064310

Title  Novel cyclotides from Hedyotis diffusa induce apoptosis and inhibit proliferation and migration of prostate cancer cells

Doi Not available

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.