Peptide Libraray

Cyclotide mela-4

General Information

DCTPep ID DCTPep00569

Peptide Name Cyclotide mela-4

Sequence GKPICGETCFKGKCYTPGCTCSYPICKKN

Sequence Length 29

UniProt ID Not available

PubChem CID Not available

Origin Melicytus latifolius

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HeLa	Human papillomavirus-related endocervical adenocarcinoma	CC50=18.73±1.82µM	Resazurin assay	24h	1
MM96L	Melanoma	CC50=2.04±0.26µM	Resazurin assay	24h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity HFF-1: CC50=5.39±0.33µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00569

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C134H213N35O39S6

Absent amino acids ADHLMQRVW

Theoretical pI 8.87

Acidic residues 1

Basic residues 5

Polar residues 17

Molecular weight (Average) 3130.74

Molecular weight (Monoisotopic) 3128.41

Common amino acids C

Net charge 4

Instability index (II) 3.06

Aliphatic index 26.90

Grand average of hydropathicity (GRAVY) -0.400

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 3355
Abs 0.1% (=1 g/l) 1.072, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.952, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 26322745

Title Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

Doi 10.1021/acschembio.5b00454

Year 2015

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available