Cyclotide mela-4

General Information


DCTPep ID  DCTPep00569

Peptide Name   Cyclotide mela-4

Sequence  GKPICGETCFKGKCYTPGCTCSYPICKKN

Sequence Length  29

UniProt ID  Not available

PubChem CID  Not available

Origin  Melicytus latifolius

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma CC50=18.73±1.82µM Resazurin assay 24h 1
MM96L Melanoma CC50=2.04±0.26µM Resazurin assay 24h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  HFF-1: CC50=5.39±0.33µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00569

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C134H213N35O39S6

Absent amino acids  ADHLMQRVW

Theoretical pI  8.87

Acidic residues  1

Basic residues  5

Polar residues  17

Molecular weight (Average)  3130.74

Molecular weight (Monoisotopic)  3128.41

Common amino acids  C

Net charge  4

Instability index (II)  3.06

Aliphatic index  26.90

Grand average of hydropathicity (GRAVY)  -0.400

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 3355
  Abs 0.1% (=1 g/l) 1.072, assuming all pairs of Cys residues form cystines
  Ext. coefficient 2980
  Abs 0.1% (=1 g/l) 0.952, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 26322745

Title  Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

Doi 10.1021/acschembio.5b00454

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.