This work is licensed under a Creative Commons Attribution 4.0 International License.
Cyclotide mela-2
General Information
DCTPep ID DCTPep00570
Peptide Name Cyclotide mela-2
Sequence GKPTCGETCFKGKCYTPGCTCSYPLCKKD
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin Melicytus latifolius
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MM96L | Melanoma | CC50=1.30±0.44µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=19.26±1.37µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>64µM
Normal (non-cancerous) Cytotoxicity HFF-1: CC50=5.86±0.38µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00570
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asp29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C132H208N34O41S6
Absent amino acids AHIMNQRVW
Theoretical pI 8.63
Acidic residues 2
Basic residues 5
Polar residues 17
Molecular weight (Average) 3119.67
Molecular weight (Monoisotopic) 3117.36
Common amino acids C
Net charge 3
Instability index (II) 3.06
Aliphatic index 13.45
Grand average of hydropathicity (GRAVY) -0.603
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 3355
Abs 0.1% (=1 g/l) 1.075, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.955, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26322745
Title Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae
Doi 10.1021/acschembio.5b00454
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.