Peptide Libraray

Cyclotide mela-1

General Information

DCTPep ID DCTPep00571

Peptide Name Cyclotide mela-1

Sequence GKYTCGETCFKGKCYTPGCTCSYPICKKD

Sequence Length 29

UniProt ID Not available

PubChem CID Not available

Origin Melicytus latifolius

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MM96L	Melanoma	CC50=2.09±0.18µM	Resazurin assay	24h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	CC50=9.83±0.78µM	Resazurin assay	24h	1

Hemolytic Activity Human erythrocytes: 50% Hemolysis>64µM

Normal (non-cancerous) Cytotoxicity HFF-1: CC50=3.07±0.15µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00571

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asp29

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C136H210N34O42S6

Absent amino acids AHLMNQRVW

Theoretical pI 8.62

Acidic residues 2

Basic residues 5

Polar residues 18

Molecular weight (Average) 3185.73

Molecular weight (Monoisotopic) 3183.37

Common amino acids C

Net charge 3

Instability index (II) 2.73

Aliphatic index 13.45

Grand average of hydropathicity (GRAVY) -0.569

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 4845
Abs 0.1% (=1 g/l) 1.521, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 1.403, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 26322745

Title Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

Doi 10.1021/acschembio.5b00454

Year 2015

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available