Peptide Libraray

Cyclotide mech-2

General Information

DCTPep ID DCTPep00574

Peptide Name Cyclotide mech-2

Sequence GLPTCGETCTLGKCNTPKCTCNWPICYKD

Sequence Length 29

UniProt ID Not available

PubChem CID Not available

Origin Melicytus chathamicus

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MM96L	Melanoma	CC50=21.94±1.90µM	Resazurin assay	24h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	CC50=36.92±3.25µM	Resazurin assay	24h	1

Hemolytic Activity Human erythrocytes: 50% Hemolysis>64µM

Normal (non-cancerous) Cytotoxicity HFF-1: CC50=12.45±0.99µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00574

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asp29

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C132H209N35O42S6

Absent amino acids AFHMQRSV

Theoretical pI 7.76

Acidic residues 2

Basic residues 3

Polar residues 17

Molecular weight (Average) 3150.68

Molecular weight (Monoisotopic) 3148.36

Common amino acids C

Net charge 1

Instability index (II) 26.75

Aliphatic index 40.34

Grand average of hydropathicity (GRAVY) -0.355

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 7365
Abs 0.1% (=1 g/l) 2.338, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 2.219, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 26322745

Title Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

Doi 10.1021/acschembio.5b00454

Year 2015

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available