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Phylloseptin-Pba
General Information
DCTPep ID DCTPep00587
Peptide Name Phylloseptin-Pba
Sequence FFSMIPKIAGGIASLVKNL
Sequence Length 19
UniProt ID Not available
PubChem CID Not available
Origin Phyllomedusa baltea
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-251MG | Astrocytoma | IC50=1.8µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=2.9µM | MTT assay | 24h | 1 |
| NCI-H460 | Lung large cell carcinoma | IC50=4.3µM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: 1.4% Hemolysis=8µg/ml; Human erythrocytes: 80% Hemolysis=128µg/ml
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=36.6µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00587
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C95H156N22O23S1
Absent amino acids CDEHQRTWY
Theoretical pI 10.00
Acidic residues 0
Basic residues 2
Polar residues 5
Molecular weight (Average) 2006.48
Molecular weight (Monoisotopic) 2005.14
Common amino acids I
Net charge 2
Instability index (II) 4
Aliphatic index 128.42
Grand average of hydropathicity (GRAVY) 1.111
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26633506
Title Phylloseptin-PBa--A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.