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Phylloseptin-PBa
General Information
DCTPep ID DCTPep00588
Peptide Name Phylloseptin-PBa
Sequence FFSMIPKIAGGIASLVKNLG
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-251MG | Astrocytoma | IC50=1.8 μM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=2.9 μM | MTT assay | 24h | 1 |
| NCI-H460 | Lung large cell carcinoma | IC50=4.3 μM | MTT assay | 24h | 1 |
Hemolytic Activity Horse red cells: Phylloseptin-PBa was associated with a relatively low cytotoxicity (1.4%) on horse erythrocytes at a concentration of 8 mg/L, but this rose to around 80% at a concentration of 128 mg/L
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=36.6 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00588
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C97H159N23O24S1
Absent amino acids CDEHQRTWY
Theoretical pI 10.00
Acidic residues 0
Basic residues 2
Polar residues 6
Molecular weight (Average) 2063.53
Molecular weight (Monoisotopic) 2062.16
Common amino acids GI
Net charge 2
Instability index (II) 4.3
Aliphatic index 122.00
Grand average of hydropathicity (GRAVY) 1.035
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26633506
Title Phylloseptin-PBa--A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.