RL2

General Information


DCTPep ID  DCTPep00592

Peptide Name   RL2

Sequence  RLLRLLRLRRLLRL

Sequence Length  14

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=4.3±0.2 μM MTT assay 24h 1
MCF-7 Invasive breast carcinoma of no special type IC50=4.5±0.3 μM MTT assay 24h 1
A549 Lung adenocarcinoma IC50=4.8±0.6 μM MTT assay 24h 1

Hemolytic Activity  Mice red blood cells: HD50>200 μM

Normal (non-cancerous) Cytotoxicity  HLF: IC50=21.4±1.3 μM; 293T: IC50=41.6±2.7 μM; HUVEC: IC50=19±1.5 μM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00592

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C84H162N32O15

Absent amino acids  ACDEFGHIKMNPQSTVWY

Theoretical pI  12.70

Acidic residues  0

Basic residues  6

Polar residues  0

Molecular weight (Average)  1860.42

Molecular weight (Monoisotopic)  1859.29

Common amino acids  L

Net charge  6

Instability index (II)  105.23

Aliphatic index  222.86

Grand average of hydropathicity (GRAVY)  0.243

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 27195657

Title  Amphipathicity Determines Different Cytotoxic Mechanisms of Lysine- or Arginine-Rich Cationic Hydrophobic Peptides in Cancer Cells

Doi 10.1021/acs.jmedchem.5b02016

Year  2016

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.