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RL2
General Information
DCTPep ID DCTPep00592
Peptide Name RL2
Sequence RLLRLLRLRRLLRL
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=4.3±0.2 μM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=4.5±0.3 μM | MTT assay | 24h | 1 |
| A549 | Lung adenocarcinoma | IC50=4.8±0.6 μM | MTT assay | 24h | 1 |
Hemolytic Activity Mice red blood cells: HD50>200 μM
Normal (non-cancerous) Cytotoxicity HLF: IC50=21.4±1.3 μM; 293T: IC50=41.6±2.7 μM; HUVEC: IC50=19±1.5 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00592
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C84H162N32O15
Absent amino acids ACDEFGHIKMNPQSTVWY
Theoretical pI 12.70
Acidic residues 0
Basic residues 6
Polar residues 0
Molecular weight (Average) 1860.42
Molecular weight (Monoisotopic) 1859.29
Common amino acids L
Net charge 6
Instability index (II) 105.23
Aliphatic index 222.86
Grand average of hydropathicity (GRAVY) 0.243
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 27195657
Title Amphipathicity Determines Different Cytotoxic Mechanisms of Lysine- or Arginine-Rich Cationic Hydrophobic Peptides in Cancer Cells
Doi 10.1021/acs.jmedchem.5b02016
Year 2016
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.