Peptide Libraray

V681 [V13E]

General Information

DCTPep ID DCTPep00601

Peptide Name V681 [V13E]

Sequence KWKSFLKTFKSAEKTVLHTALKAISS

Sequence Length 26

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
A549	Lung adenocarcinoma	IC50=109.68µM	MTT assay	24h	1
SGC-7901	Human papillomavirus-related endocervical adenocarcinoma	IC50=92.04µM	MTT assay	24h	1

Hemolytic Activity Human erythrocytes:<5% Hemolysis=>500µM

Normal (non-cancerous) Cytotoxicity 3T3-L1:50% Cell death>125µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00601

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C138H225N35O36

Absent amino acids CDGMNPQRY

Theoretical pI 10.30

Acidic residues 1

Basic residues 7

Polar residues 7

Molecular weight (Average) 2950.52

Molecular weight (Monoisotopic) 2948.69

Common amino acids K

Net charge 6

Instability index (II) 6.56

Aliphatic index 82.69

Grand average of hydropathicity (GRAVY) -0.200

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.864

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 27363513

Title Specificity and mechanism of action of alpha-helical membrane-active peptides interacting with model and biological membranes by single-molecule force spectroscopy

Doi 10.1038/srep29145

Year 2016

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available