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P144
General Information
DCTPep ID DCTPep00604
Peptide Name P144
Sequence TSLDASIWAMMQNA
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin derived from the extracellular sequence of the human TGF-β type III receptor
Type Native peptide
Classification
ACP Tumor active peptide Cancer targeted peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-87MG ATCC | Glioblastoma | IC50=16.34±1.47 µg/mL | MTT assay | 48h | 1 |
| A172 | Glioblastoma | IC50=21.96±3.68 µg/mL | MTT assay | 48h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Transforming Growth Factor beta
Affinity Not available
Mechanism P144 induced an upregulation of SMAD7 and downregulation of SKI through inhibition of the TGF-β pathway.
Structure Information
PDB ID Not available
Predicted Structure DCTPep00604
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C65H103N17O22S2
Absent amino acids CEFGHKPRVY
Theoretical pI 3.80
Acidic residues 1
Basic residues 0
Polar residues 4
Molecular weight (Average) 1538.75
Molecular weight (Monoisotopic) 1537.69
Common amino acids A
Net charge -1
Instability index (II) -8.89
Aliphatic index 77.14
Grand average of hydropathicity (GRAVY) 0.271
Half Life
7.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.574
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 27473823
Title P144, a Transforming Growth Factor beta inhibitor peptide, generates antitumoral effects and modifies SMAD7 and SKI levels in human glioblastoma cell lines
Doi 10.1016/j.canlet.2016.07.029
Year 2016
Literature 2
Pubmed ID 12946101
Title A synthetic peptide from transforming growth factor beta type III receptor inhibits liver fibrogenesis in rats with carbon tetrachloride liver injury
Doi 10.1016/s1043-4666(03)00101-7
Year 2003
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.