Peptide Libraray

NaD1 (1-24)-NaD2 (25-29)-NaD1 (30-47)

General Information

DCTPep ID DCTPep00609

Peptide Name NaD1 (1-24)-NaD2 (25-29)-NaD1 (30-47)

Sequence RECKTESNTFPGICITKPPCRKACLTEGFTDGHCSKILRRCLCTKPC

Sequence Length 47

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
U-937	Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia	44.6±8.5% Cell death=10µM	PI uptake assay	30min	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00609

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys23<--->Cys47; Cys14<---Cys34; Cys20<--->Cys41; Cys24<--->Cys43

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C220H367N67O65S8

Absent amino acids MQVWY

Theoretical pI 8.92

Acidic residues 4

Basic residues 10

Polar residues 20

Molecular weight (Average) 5247.22

Molecular weight (Monoisotopic) 5243.52

Common amino acids C

Net charge 6

Instability index (II) 75.28

Aliphatic index 51.91

Grand average of hydropathicity (GRAVY) -0.411

Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 500
Abs 0.1% (=1 g/l) 0.095, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 27503651

Title Nicotiana alata Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant

Doi 10.1128/AAC.01479-16

Year 2016

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available