Class I defensin, NaD2

General Information


DCTPep ID  DCTPep00610

Peptide Name   Class I defensin, NaD2

Sequence  RTCESQSHRFKGPCARDSNCATVCLTEGFSGGDCRGFRRRCFCTRPC

Sequence Length  47

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

Cancer therapy related peptides



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
U-937 Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia 5.9±1.3% Cell death=10 µM PI uptake assay 30min 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00610

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys23<--->Cys47; Cys14<---Cys34; Cys20<--->Cys41; Cys24<--->Cys43

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C212H338N76O66S8

Absent amino acids  IMWY

Theoretical pI  8.99

Acidic residues  4

Basic residues  10

Polar residues  22

Molecular weight (Average)  5263.97

Molecular weight (Monoisotopic)  5260.32

Common amino acids  CR

Net charge  6

Instability index (II)  67.03

Aliphatic index  18.72

Grand average of hydropathicity (GRAVY)  -0.691

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 500
  Abs 0.1% (=1 g/l) 0.095, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 27503651

Title  Nicotiana alata Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant

Doi 10.1128/AAC.01479-16

Year  2016

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.