This work is licensed under a Creative Commons Attribution 4.0 International License.
Flower-specific defensin / NaD1
General Information
DCTPep ID DCTPep00612
Peptide Name Flower-specific defensin / NaD1
Sequence RECKTESNTFPGICITKPPCRKACISEKFTDGHCSKILRRCLCTKPC
Sequence Length 47
UniProt ID Not available
PubChem CID Not available
Origin Nicotiana alata
Type Native peptide
Classification
Cancer therapy related peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| PC-3 | Prostate carcinoma | 100% Killing=25 µM | MTT assay | 48h | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | 100% Killing=25 µM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: 1.5% Hemolysis=50 µM
Normal (non-cancerous) Cytotoxicity Human dermal fibroblasts: 100% Killing=25 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00612
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys47; Cys14<--->Cys34; Cys20<--->Cys41; Cys24<--->Cys43
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C223H374N68O65S8
Absent amino acids MQVWY
Theoretical pI 9.08
Acidic residues 4
Basic residues 11
Polar residues 19
Molecular weight (Average) 5304.32
Molecular weight (Monoisotopic) 5300.58
Common amino acids C
Net charge 7
Instability index (II) 71.18
Aliphatic index 51.91
Grand average of hydropathicity (GRAVY) -0.472
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 500
Abs 0.1% (=1 g/l) 0.094, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 27503651
Title Nicotiana alata Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant
Year 2016
Literature 2
Pubmed ID 31031739
Title Salt-Tolerant Antifungal and Antibacterial Activities of the Corn Defensin ZmD32
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.