MCoTI-II

General Information


DCTPep ID  DCTPep00624

Peptide Name   MCoTI-II

Sequence  CPKILKKCRRDSDCPGACICRGNGYCGSGSDGGV

Sequence Length  34

UniProt ID  P82409 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

Cancer targeted peptides Induce apoptosis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HT-29 Colon adenocarcinoma IC50>100 μM MTT assay 48h 1
MCF-7 Invasive breast carcinoma of no special type IC50>100 μM MTT assay 48h 1
PC-3 Prostate carcinoma IC50>100 μM MTT assay 48h 1

Hemolytic Activity  Human red blood cells: no hemolytic activity(as show in Fig.4)

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50>100 μM

Target  pigment epithelium-derived factor; somatostatin

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  2PO8 

Predicted Structure  DCTPep00624

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C138H231N47O46S6

Absent amino acids  EFHMQTW

Theoretical pI  8.66

Acidic residues  3

Basic residues  6

Polar residues  18

Molecular weight (Average)  3477

Molecular weight (Monoisotopic)  3474.55

Common amino acids  G

Net charge  3

Instability index (II)  46.36

Aliphatic index  45.88

Grand average of hydropathicity (GRAVY)  -0.444

Half Life 
  1.2 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1865
  Abs 0.1% (=1 g/l) 0.536, assuming all pairs of Cys residues form cystines
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 0.429, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 27734947

Title  Dual-targeting anti-angiogenic cyclic peptides as potential drug leads for cancer therapy

Doi 10.1038/srep35347

Year  2016

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.