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LL-37 KR-12
General Information
DCTPep ID DCTPep00648
Peptide Name LL-37 KR-12
Sequence KRIVQRIKDFLR
Sequence Length 12
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| CaCo-2 | Colon adenocarcinoma | EC50=125µg/ml | MTT assay | 24h | 1 |
| A431 | Skin squamous cell carcinoma | EC50=31.2µg/ml | MTT assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>800µM; 10.6% Hemolysis=100µM; <5% Hemolysis=256µg/ml; Rabbit erythrocytes: 73% Hemolysis=125µg/ml
Normal (non-cancerous) Cytotoxicity NIH 3T3: EC50=0.8µg/ml; HaCat: IC50=74.6µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C71H126N24O16
Absent amino acids ACEGHMNPSTWY
Theoretical pI 11.72
Acidic residues 1
Basic residues 5
Polar residues 0
Molecular weight (Average) 1571.93
Molecular weight (Monoisotopic) 1570.98
Common amino acids R
Net charge 4
Instability index (II) 31.95
Aliphatic index 121.67
Grand average of hydropathicity (GRAVY) -0.708
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 27907988
Title KR12 peptide associated with cyclodextrin: Antimicrobial and antitumor activities
Year 2016
Literature 2
Pubmed ID 24105706
Title Short KR-12 analogs designed from human cathelicidin LL-37 possessing both antimicrobial and antiendotoxic activities without mammalian cell toxicity
Doi 10.1002/psc.2552
Year 2013
Literature 3
Pubmed ID 24307932
Title Structural location determines functional roles of the basic amino acids of KR-12, the smallest antimicrobial peptide from human cathelicidin LL-37
Year 2013
Literature 4
Pubmed ID 32019109
Title Lipidated Analogs of the LL-37-Derived Peptide Fragment KR12-Structural Analysis, Surface-Active Properties and Antimicrobial Activity
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.