This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-1Tb
General Information
DCTPep ID DCTPep00695
Peptide Name Temporin-1Tb
Sequence LLPIVGNLLKSLL
Sequence Length 13
UniProt ID Not available
PubChem CID Not available
Origin Rana temporaria
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | 10% Killing=96 µM | PI flow cytometric assay | 24h | 1 |
| HeLa S3 | Colon adenocarcinoma | IC50=93 µg/ml | MTT assay | 24h | 2 |
Hemolytic Activity Human erythrocytes: 5% Hemolysis=32 µM
Normal (non-cancerous) Cytotoxicity Human PBMC: 10% Killing=96 µM; Vero cells: 50% Cytotoxicity=90.4 µg/ml; HEK293: 50% Cytotoxicity=84 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00695
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C67H121N15O16
Absent amino acids ACDEFHMQRTWY
Theoretical pI 8.75
Acidic residues 0
Basic residues 1
Polar residues 3
Molecular weight (Average) 1392.79
Molecular weight (Monoisotopic) 1391.91
Common amino acids L
Net charge 1
Instability index (II) -2.08
Aliphatic index 232.31
Grand average of hydropathicity (GRAVY) 1.638
Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28443279
Title Analogs of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide
Year 2017
Literature 2
Pubmed ID 29483113
Title The Amphibian Antimicrobial Peptide Temporin B Inhibits In Vitro Herpes Simplex Virus 1 Infection
Year 2018
Literature 3
Pubmed ID 20606068
Title Anti-Pseudomonas activity of frog skin antimicrobial peptides in a Caenorhabditis elegans infection model: a plausible mode of action in vitro and in vivo
Year 2010
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.