B7

General Information


DCTPep ID  DCTPep00712

Peptide Name   B7

Sequence  KKLFKKILKY

Sequence Length  10

UniProt ID  Not available

PubChem CID  Not available

Origin  antimicrobial peptides

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=73.7±6.1 μM MTT assay 48h 1
MCF-7 Invasive breast carcinoma of no special type IC50=77.3±5.6 μM MTT assay 48h 1
MCF-7/ADM Invasive breast carcinoma of no special type IC50=97.3±11.4 μM MTT assay 48h 1
K562/Adr Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia IC50>100 μM MTT assay 48h 1

Hemolytic Activity  Rabbit red blood cells: very low hemolytic activity even at the concentration of 160 μm(as show in fig.3)

Normal (non-cancerous) Cytotoxicity  GES-1: IC50>100 μM

Target  Not available

Affinity  Not available

Mechanism  disrupt the cell membrane, trigger the cytochrome C release into cytoplasm, and ultimately lead to apoptosis



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C66H113N15O12

Absent amino acids  ACDEGHMNPQRSTVW

Theoretical pI  10.30

Acidic residues  0

Basic residues  5

Polar residues  1

Molecular weight (Average)  1308.72

Molecular weight (Monoisotopic)  1307.87

Common amino acids  K

Net charge  5

Instability index (II)  -12.24

Aliphatic index  117.00

Grand average of hydropathicity (GRAVY)  -0.590

Half Life 
  1.3 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  3 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 1.139

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28524273

Title  Synthesis and biological evaluation of novel peptides based on antimicrobial peptides as potential agents with antitumor and multidrug resistance-reversing activities

Doi 10.1111/cbdd.13023

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.