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B7
General Information
DCTPep ID DCTPep00712
Peptide Name B7
Sequence KKLFKKILKY
Sequence Length 10
UniProt ID Not available
PubChem CID Not available
Origin antimicrobial peptides
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=73.7±6.1 μM | MTT assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=77.3±5.6 μM | MTT assay | 48h | 1 |
| MCF-7/ADM | Invasive breast carcinoma of no special type | IC50=97.3±11.4 μM | MTT assay | 48h | 1 |
| K562/Adr | Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia | IC50>100 μM | MTT assay | 48h | 1 |
Hemolytic Activity Rabbit red blood cells: very low hemolytic activity even at the concentration of 160 μm(as show in fig.3)
Normal (non-cancerous) Cytotoxicity GES-1: IC50>100 μM
Target Not available
Affinity Not available
Mechanism disrupt the cell membrane, trigger the cytochrome C release into cytoplasm, and ultimately lead to apoptosis
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C66H113N15O12
Absent amino acids ACDEGHMNPQRSTVW
Theoretical pI 10.30
Acidic residues 0
Basic residues 5
Polar residues 1
Molecular weight (Average) 1308.72
Molecular weight (Monoisotopic) 1307.87
Common amino acids K
Net charge 5
Instability index (II) -12.24
Aliphatic index 117.00
Grand average of hydropathicity (GRAVY) -0.590
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 1.139
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28524273
Title Synthesis and biological evaluation of novel peptides based on antimicrobial peptides as potential agents with antitumor and multidrug resistance-reversing activities
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.