This work is licensed under a Creative Commons Attribution 4.0 International License.
BP100
General Information
DCTPep ID DCTPep00713
Peptide Name BP100
Sequence KKLFKKILKYL
Sequence Length 11
UniProt ID Not available
PubChem CID 91105412
Origin antimicrobial peptides
Type Synthetic peptide
Classification
ACP Tumor active peptide Cell-penetrating peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7/ADM | Invasive breast carcinoma of no special type | IC50=25.3±2.5 μM | MTT assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=27.6±3.0 μM | MTT assay | 48h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=30.4±2.7 μM | MTT assay | 48h | 1 |
| K562/Adr | Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia | IC50=32.6±3.4 μM | MTT assay | 48h | 1 |
| CAPAN-1 | Pancreatic ductal adenocarcinoma | IC50=57.7 ± 13.3 µM | MTT assay | 48 h | 2 |
Hemolytic Activity Human erythrocytes: 22 ± 2.8% Hemolysis=150µM
Normal (non-cancerous) Cytotoxicity GES-1: IC50=57.9±5.2 µM; NIH 3T3: IC50=62.5 ± 6.4 µM
Target Not available
Affinity Not available
Mechanism disrupt the cell membrane, trigger the cytochrome C release into cytoplasm, and ultimately lead to apoptosis
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C72H124N16O13
Absent amino acids ACDEGHMNPQRSTVW
Theoretical pI 10.30
Acidic residues 0
Basic residues 5
Polar residues 1
Molecular weight (Average) 1421.88
Molecular weight (Monoisotopic) 1420.95
Common amino acids K
Net charge 5
Instability index (II) -10.22
Aliphatic index 141.82
Grand average of hydropathicity (GRAVY) -0.191
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 1.048
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28524273
Title Synthesis and biological evaluation of novel peptides based on antimicrobial peptides as potential agents with antitumor and multidrug resistance-reversing activities
Year 2017
Literature 2
Pubmed ID 24480922
Title Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.