I-1

General Information


DCTPep ID  DCTPep00733

Peptide Name   I-1

Sequence  VKRFKKFFRKLKKSVL

Sequence Length  16

UniProt ID  Not available

PubChem CID  Not available

Origin  cationic host defense peptides

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type IC50=12.7±2.5 μM MTT assay 48h 1
K562/ADM Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia IC50=19.3±0.8 μM MTT assay 48h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=26.5±0.9 μM MTT assay 48h 1
MCF-7/Taxol Invasive breast carcinoma of no special type IC50=31.1±5.1 μM MTT assay 48h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=9.8±1.5 μM MTT assay 48h 1

Hemolytic Activity  Rabbit red blood cells: modest hemolytic activity(as show in Fig.2)

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50=50.62±6.1 μM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00733

(Please note that there is the predicted structure, predicted by AlphaFold)

SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C100H170N28O18

Absent amino acids  ACDEGHIMNPQTWY

Theoretical pI  12.05

Acidic residues  0

Basic residues  8

Polar residues  1

Molecular weight (Average)  2052.63

Molecular weight (Monoisotopic)  2051.32

Common amino acids  K

Net charge  8

Instability index (II)  7.94

Aliphatic index  85.00

Grand average of hydropathicity (GRAVY)  -0.550

Half Life 
  100 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28664269

Title  Pro-apoptotic cationic host defense peptides rich in lysine or arginine to reverse drug resistance by disrupting tumor cell membrane

Doi 10.1007/s00726-017-2453-y

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.