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I-9
General Information
DCTPep ID DCTPep00735
Peptide Name I-9
Sequence VKRFKLFFRKLKSV
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin cationic host defense peptides
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=16.5±0.4 μM | MTT assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=31.1±2.2 μM | MTT assay | 48h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=33.4±1.1 μM | MTT assay | 48h | 1 |
| MCF-7/Taxol | Invasive breast carcinoma of no special type | IC50=43.6±1.1 μM | MTT assay | 48h | 1 |
| K562/ADM | Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia | IC50=55.6±3.3 μM | MTT assay | 48h | 1 |
Hemolytic Activity Rabbit red blood cells: modest hemolytic activity(as show in Fig.2)
Normal (non-cancerous) Cytotoxicity HUVEC: IC50=72.37±2.7 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00735
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C88H146N24O16
Absent amino acids ACDEGHIMNPQTWY
Theoretical pI 12.03
Acidic residues 0
Basic residues 6
Polar residues 1
Molecular weight (Average) 1796.28
Molecular weight (Monoisotopic) 1795.13
Common amino acids K
Net charge 6
Instability index (II) 1.59
Aliphatic index 97.14
Grand average of hydropathicity (GRAVY) -0.071
Half Life
100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28664269
Title Pro-apoptotic cationic host defense peptides rich in lysine or arginine to reverse drug resistance by disrupting tumor cell membrane
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.