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Brevinin-1ITa
General Information
DCTPep ID DCTPep00753
Peptide Name Brevinin-1ITa
Sequence IVPFLLGMVPKLVCLITKKC
Sequence Length 20
UniProt ID C0HL47
PubChem CID Not available
Origin skin secretions of the Italian stream frog Rana italica (Ranidae)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | LC50=18 μM | CellTiter-Glo Lumines- cent Cell Viability assay | 24h | 1 |
| HT-29 | Colon adenocarcinoma | LC50=18 μM | CellTiter-Glo Lumines- cent Cell Viability assay | 24h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | LC50=8 μM | CellTiter-Glo Lumines- cent Cell Viability assay | 24h | 1 |
Hemolytic Activity mouse erythrocytes: LC50=7 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00753
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C105H183N23O22S3
Absent amino acids ADEHNQRSWY
Theoretical pI 9.39
Acidic residues 0
Basic residues 3
Polar residues 4
Molecular weight (Average) 2215.93
Molecular weight (Monoisotopic) 2214.31
Common amino acids L
Net charge 3
Instability index (II) 39.53
Aliphatic index 160.50
Grand average of hydropathicity (GRAVY) 1.525
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.056, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28699258
Title Cytotoxic peptides with insulin-releasing activities from skin secretions of the Italian stream frog Rana italica (Ranidae)
Doi 10.1002/psc.3025
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.