[Y14W]cGm

General Information


DCTPep ID  DCTPep00757

Peptide Name   [Y14W]cGm

Sequence  GCRRLCYKQRCVTWCRGR

Sequence Length  18

UniProt ID  Not available

PubChem CID  Not available

Origin  Redesigned Spider Peptide

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia CC50=2.7 ± 0.1 µM Resazurin assay 18-24h 1
CRL-1739 Gastric adenocarcinoma CC50=30.3 ± 1.1 µM Resazurin assay 18-24h 1
MM96L Melanoma CC50=4.0 ± 0.1 µM Resazurin assay 18-24h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma CC50=50.4 ± 2.4 µM Resazurin assay 18-24h 1

Hemolytic Activity  Human red blood cells (hRBCs): hemolysis(%)=50.8±4.7 with 64 μM of peptide

Normal (non-cancerous) Cytotoxicity  HFF-1: CC50=68.4 ± 9.6 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00757

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys2<--->Cys15; Cys6<--->Cys11

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C92H154N36O22S4

Absent amino acids  ADEFHIMNPS

Theoretical pI  10.16

Acidic residues  0

Basic residues  6

Polar residues  8

Molecular weight (Average)  2244.7

Molecular weight (Monoisotopic)  2243.09

Common amino acids  R

Net charge  6

Instability index (II)  32.45

Aliphatic index  37.78

Grand average of hydropathicity (GRAVY)  -0.867

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 7240
  Abs 0.1% (=1 g/l) 3.225, assuming all pairs of Cys residues form cystines
  Ext. coefficient 6990
  Abs 0.1% (=1 g/l) 3.114, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28741926

Title  Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties

Doi 10.1021/acschembio.7b00459

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.