This work is licensed under a Creative Commons Attribution 4.0 International License.
[L5W]cGm
General Information
DCTPep ID DCTPep00761
Peptide Name [L5W]cGm
Sequence GCRRWCYKQRCVTYCRGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=1.0 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=17.1 ± 0.7 µM | Resazurin assay | 18-24h | 1 |
| CRL-1739 | Gastric adenocarcinoma | CC50=18.3 ± 1.4 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | CC50=4.0 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=40.1±3.3 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity HFF-1: CC50=25.0 ± 2.9 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00761
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C95H152N36O23S4
Absent amino acids ADEFHILMNPS
Theoretical pI 9.93
Acidic residues 0
Basic residues 6
Polar residues 9
Molecular weight (Average) 2294.72
Molecular weight (Monoisotopic) 2293.07
Common amino acids R
Net charge 6
Instability index (II) 72.62
Aliphatic index 16.11
Grand average of hydropathicity (GRAVY) -1.150
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 3.804, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.695, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.