This work is licensed under a Creative Commons Attribution 4.0 International License.
[G1K,L5Y,K8R]cGm
General Information
DCTPep ID DCTPep00765
Peptide Name [G1K,L5Y,K8R]cGm
Sequence KCRRYCYRQRCVTYCRGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=1.3 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | CC50=15.2 ± 1.9 µM | Resazurin assay | 18-24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | CC50=15.7 ± 1.1 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | CC50=2.0 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=20.9 ± 1.4 µM | Resazurin assay | 18-24h | 1 |
| CRL-1739 | Gastric adenocarcinoma | CC50=26.5 ± 2.4 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=26.3±5.7 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=10.4±1.0µM, testing time is 18-24h; HFF-1: CC50=14.7 ± 1.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00765
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C97H160N38O24S4
Absent amino acids ADEFHILMNPSW
Theoretical pI 10.10
Acidic residues 0
Basic residues 7
Polar residues 9
Molecular weight (Average) 2370.82
Molecular weight (Monoisotopic) 2369.14
Common amino acids R
Net charge 7
Instability index (II) 24.76
Aliphatic index 16.11
Grand average of hydropathicity (GRAVY) -1.400
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 4720
Abs 0.1% (=1 g/l) 1.991, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 1.885, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.