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L-K6
General Information
DCTPep ID DCTPep00775
Peptide Name L-K6
Sequence IKKILSKIKKLLK
Sequence Length 13
UniProt ID Not available
PubChem CID Not available
Origin some cationic antimicrobial peptides
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50=23 μM | MTT assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=31 μM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=34 μM | MTT assay | 6h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=38 μM | MTT assay | 1h | 1 |
Hemolytic Activity Rabbit red blood cells: modest hemolytic activity(as show in Fig.2)
Normal (non-cancerous) Cytotoxicity HUVEC: IC50=65.88±4.5 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00775
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C75H145N19O15
Absent amino acids ACDEFGHMNPQRTVWY
Theoretical pI 10.70
Acidic residues 0
Basic residues 6
Polar residues 1
Molecular weight (Average) 1553.09
Molecular weight (Monoisotopic) 1552.12
Common amino acids K
Net charge 6
Instability index (II) -15.14
Aliphatic index 180.00
Grand average of hydropathicity (GRAVY) 0.054
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28811617
Title Cell surface binding, uptaking and anticancer activity of L-K6, a lysine/leucine-rich peptide, on human breast cancer MCF-7 cells
Doi 10.1038/s41598-017-08963-2
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.