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HD5
General Information
DCTPep ID DCTPep00805
Peptide Name HD5
Sequence ATCYCRTGRCATRESLSGVCEISGRLYRLCCR
Sequence Length 32
UniProt ID Not available
PubChem CID Not available
Origin Homo sapiens
Type Native peptide
Classification
Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| JLTRG (Jurkat LTR-GFP Cells) | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | Not active up to 50 µg/ml | CCK-8 assay | 36h | 1 |
Hemolytic Activity Human erythrocytes: NA; Mouse erythrocytes: 20% Hemolysis=25 µg/ml; 33% Hemolysis=50 µg/ml; 40% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity HaCat: 10% Killing=150 µg/ml; ARPE-19: 50% Cytotoxicity=24 µM; CaSki: Not active up to 50 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00805
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C144H244N50O45S6
Absent amino acids DFHKMNPQW
Theoretical pI 8.96
Acidic residues 2
Basic residues 6
Polar residues 17
Molecular weight (Average) 3588.19
Molecular weight (Monoisotopic) 3585.67
Common amino acids CR
Net charge 4
Instability index (II) 13.79
Aliphatic index 64.06
Grand average of hydropathicity (GRAVY) -0.113
Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 3355
Abs 0.1% (=1 g/l) 0.935, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.831, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23269800
Title Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites
Year 2013
Literature 2
Pubmed ID 29158275
Title A Simplified Derivative of Human Defensin 5 with Potent and Efficient Activity against Multidrug-Resistant Acinetobacter baumannii
Year 2018
Literature 3
Pubmed ID 32209394
Title The human α-defensin-derived peptide HD5(1-9) inhibits cellular attachment and entry of human cytomegalovirus
Doi 10.1016/j.antiviral.2020.104779
Year 2020
Literature 4
Pubmed ID 26206286
Title Antimicrobial activity of human α-defensin 5 and its linear analogs: N-terminal fatty acylation results in enhanced antimicrobial activity of the linear analogs
Doi 10.1016/j.peptides.2015.07.009
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.