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PE-BBI
General Information
DCTPep ID DCTPep00839
Peptide Name PE-BBI
Sequence GALKGCWTKSIPPKPCK
Sequence Length 17
UniProt ID Not available
PubChem CID Not available
Origin skin secretion of Pelophylax esculentus (PE) named PE-BBI
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | IC50=35.4 μM | MTT assay | 24h | 1 |
| DLD-1 | Colon adenocarcinoma | IC50=50.1 μM | MTT assay | 24h | 1 |
| DKs-8 | Colon adenocarcinoma | IC50=9.8 μM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HKE3: IC50=50.2 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00839
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C82H136N22O20S2
Absent amino acids DEFHMNQRVY
Theoretical pI 9.70
Acidic residues 0
Basic residues 4
Polar residues 6
Molecular weight (Average) 1814.24
Molecular weight (Monoisotopic) 1812.97
Common amino acids K
Net charge 4
Instability index (II) 0.28
Aliphatic index 51.76
Grand average of hydropathicity (GRAVY) -0.500
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 3.100, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.032, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30267012
Title Identification and pharmaceutical evaluation of novel frog skin-derived serine proteinase inhibitor peptide-PE-BBI (Pelophylax esculentus Bowman-Birk inhibitor) for the potential treatment of cancer
Doi 10.1038/s41598-018-32947-5
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.