This work is licensed under a Creative Commons Attribution 4.0 International License.
LyeTxI-b
General Information
DCTPep ID DCTPep00850
Peptide Name LyeTxI-b
Sequence IWLTALKFLGKNLGKLAKQQLAKL
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Lycosa erythrognatha spider venom
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-373MG ATCC | Astrocytoma | IC50=20.94±5.18 μM | MTT assay | 48h | 1 |
| U-87MG ATCC | Glioblastoma | IC50=29.20±7.96 μM | MTT assay | 48h | 1 |
| SH-SY5Y | Neuroblastoma | IC50=93.80±2.17 μM | MTT assay | 48h | 1 |
Hemolytic Activity hRBC(human blood red cells): IC50=155±13.04 μM (testing time is 48 h)
Normal (non-cancerous) Cytotoxicity Vero(African green monkey kidney): IC50≥100 μM (testing time is 48 h); PBMC(Peripheral Blood Mononuclear Cells): IC50=4.16±1.94 μM (testing time is 48 h); GM00637: IC50 ≥ 100 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00850
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C129H219N33O29
Absent amino acids CDEHMPRSVY
Theoretical pI 10.60
Acidic residues 0
Basic residues 5
Polar residues 4
Molecular weight (Average) 2696.36
Molecular weight (Monoisotopic) 2694.67
Common amino acids L
Net charge 5
Instability index (II) 14.93
Aliphatic index 142.50
Grand average of hydropathicity (GRAVY) 0.287
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.040
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30449002
Title The synthetic peptide LyeTxI-b derived from Lycosa erythrognatha spider venom is cytotoxic to U-87 MG glioblastoma cells
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.