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PM II
General Information
DCTPep ID DCTPep00855
Peptide Name PM II
Sequence RRWCFRVCYKGFCYRKCR
Sequence Length 18
UniProt ID P14216
PubChem CID Not available
Origin horseshoe crab Limulus polyphemus
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | IC50=10.8±1.9 μM | MTT assay | 48h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=16.4±1.3 μM | MTT assay | 48h | 1 |
| SK-BR-3 | Breast adenocarcinoma | IC50=17.3±0.6 μM | MTT assay | 48h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=7.2±0.5 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human red blood cells (hBRC): only ~30%, even at the maximal concentration of 100 µM
Normal (non-cancerous) Cytotoxicity HEK 293T: IC50=11.3±1.6 μM; HEF: IC50=9.7±0.2 μM; NHA: IC50=18.3±1.4 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00855
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C108H164N36O21S4
Absent amino acids ADEHILMNPQST
Theoretical pI 10.10
Acidic residues 0
Basic residues 7
Polar residues 7
Molecular weight (Average) 2430.96
Molecular weight (Monoisotopic) 2429.18
Common amino acids R
Net charge 7
Instability index (II) 58.98
Aliphatic index 16.11
Grand average of hydropathicity (GRAVY) -0.800
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 3.591, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.488, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30486233
Title Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.